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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 133

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Progesterone

1.259

0.01

0.063

95% aq. Ethanol

Human

Dermatomed to 0.6mm

Breast/Abdomen

0.2

Static

isotonic saline % 1.5% BSA

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
			2.42	8.32		9.03		0.71	0.18				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2.42

8.32

9.03

0.71

0.18

NOTES

They added 0.2microCi/microlitre and 4nmol/microlitre. Specific Activity was 85Ci/mmol. Data was given +/- SEM. I have converted this to SD for the database.

REFERENCE

Role Of Transepidermal And Transfollicular Routes In Percutaneous Absorption Of Steriods: In Vitro Studies On Human Skin (1994) Skin Pharmacol., 7 :237-244