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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 134

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Progesterone1.2590.010.06395% aq. EthanolHumanDermatomed to 0.6mmBreast/Abdomen Scar Tissue0.2Staticisotonic saline % 1.5% BSA37Receptor Fluid882Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
   1.264.31 4.54 0.230.14   4 

NOTES

They added 0.2microCi/microlitre and 4nmol/microlitre. Specific Activity was 85Ci/mmol. Data was given +/- SEM. I have converted this to SD for the database.

REFERENCE

Role Of Transepidermal And Transfollicular Routes In Percutaneous Absorption Of Steriods: In Vitro Studies On Human Skin (1994) Skin Pharmacol., 7 :237-244