IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 134
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Progesterone
1.259
0.01
0.063
95% aq. Ethanol
Human
Dermatomed to 0.6mm
Breast/Abdomen Scar Tissue
0.2
Static
isotonic saline % 1.5% BSA
37
Receptor Fluid
8
8
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
1.26
4.31
4.54
0.23
0.14
4
NOTES
They added 0.2microCi/microlitre and 4nmol/microlitre. Specific Activity was 85Ci/mmol. Data was given +/- SEM. I have converted this to SD for the database.
REFERENCE
Role Of Transepidermal And Transfollicular Routes In Percutaneous Absorption Of Steriods: In Vitro Studies On Human Skin (1994) Skin Pharmacol., 7 :237-244