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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1355

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Acetyl ethyl tetramethyl tetralin

See Notes

Petrolatum

Rat

Dermatomed to 0.35mm

Back

1.13

Static

50% aq. Glycerol

Water Bath

120

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								0.14	0.05				3

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.14

0.05

NOTES

Female Osborne-Mendel Rats were used. Results were given +/- SE. I have converted this to SD for this database. 30mg of petrolatum was added

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies III: Hydrophobic Compounds (1984) J. Pharm. Sci., 73 :1255-1258