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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1374

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Urea

0.04

Water

Mouse

Not Given

Dorsal

Flow-through

Saline & 0.01M unlabelled urea

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												1.1	2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

1.1

NOTES

Hairless Mouse Skin was used. More experimental detail to be obtained from Ackermann C et al. (1985) Percutaneous Absorption of Urea Int. J. cosmet. Sci. 7 251-261

REFERENCE

Percutaneous Absorption Of Urea And Water In Vitro In Closed And Open Diffusion Cells (1988) S. African J. Sci., 84 :440-441