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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1385

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
17a-Hydroxyprogesterone0.121Infinite 40% aq. PEG 400MouseFull Thickness SkinAbdomen Static40% aq. PEG 40037Water Bath  2HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.290.05  4.55

NOTES

Female hairless Mouse skin was used. The saturated solution of the drug was added with excess drug crystals to ensure a saturated concetration throughout the course of the experiment.

REFERENCE

Drug Permeation Across The Skin: Effect Of Penetrant Hydrophilicity (1987) J. Pharm. Sci., 76 :123-126