EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

 University of Newcastle homepage

In Vitro Study No: 139

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Testosterone0.50.010.0025AcetoneMouseFull Thickness SkinNot Given2Flow-throughoxygenated culture medium Not Given16162Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        70.53.66     

NOTES

These were additional studies to the main focus of the paper.

REFERENCE

Skin Absorption And Cutaneous First Pass Metabolism Of Topical Steroids: In Vitro Studies With Mouse Skin In Organ Culture. (1987) Occup. Environ. Med., 241 :482-487