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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 140

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Testosterone0.50.10.025AcetoneMouseFull Thickness SkinNot Given2Flow-throughoxygenated culture medium Not Given16162Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        442.4     

NOTES

These were additional studies to the main focus of the paper.

REFERENCE

Skin Absorption And Cutaneous First Pass Metabolism Of Topical Steroids: In Vitro Studies With Mouse Skin In Organ Culture. (1987) Occup. Environ. Med., 241 :482-487