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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1401

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Anthracene

0.17

0.096

0.0093

Hexane:Acetone (7:1)

Rat

Dermatomed to 0.35mm

Dorsal

1.77

Static

6% aq. Volpo-20

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								39.8	2.9				4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

39.8

2.9

NOTES

Female Sprague-Dawley Rats were used. The concentration was given as ABOUT 0.2mg/ml, however this figure does not fit in with the other figures given. So I have changed it to 0.17 as this was the only figure given as an approximate. % Absorption is calculated from the amount recovered in the receptor fluid.

REFERENCE

Percutaneous Absorption Of Anthracene In The Rat: Comparison Of In Vivo And In Vitro Results (1986) Toxicol. Ind. Health, 2 :79-84