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In Vitro Study No: 1408

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Testosterone 0.005 AcetoneRatDermatomed to 0.35mmDorsal0.32Flow-throughNormal Saline Not Given24722Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
99.8   8.71   35.44.83   14 

NOTES

Osborne Mendel Rats 3-6 months old were used. The concentration was betweeen 1 and 6 microg/cm2. The skin surface was washed a 24 with a 0.5% solution of a common dishwashing detergent. The amount recovered in the skin was from the SC, epidermis and dermis including the skin that was surrounding the exposed area. 55.4% was recovered at the surface wash at 24h. Results were given as +/- SEM. I have converted these to SD for this database.

REFERENCE

The Influence of Solubility, Protein Binding, and Percutaneous Absorption On Reservoir Formation In The Skin (1988) J. Soc. Cosmet. Chem., 39 :169-177