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In Vitro Study No: 1434

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
2-Ethoxyethanol30.10.469WaterHumanDermatomed to 0.5mmBreast0.64Flow-throughEagles MEM, sodium hydrogen carbonate and gentamycin32Receptor Fluid24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
96.299.3900.630.28014.4 14.124.02   5 

NOTES

Charcoal filters were placed above the donor chamber to trap any volatised solution. In this study 81.37% of the applied dose was recovered in the charcoal filters. No further absorption into the receptor fluid was measured after about 5h, presumably due to the evaporation of the dose material. Results were given +/- SEM. I have converted these to SD for the purpose of this database.

REFERENCE

Effects Of Experimental Conditions On Absorption Of Glycol Ethers Through Human Skin In Vitro (2002) Int. Arch. Occup. Environ. Health, 75 :519-527