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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1453

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Nicotinic Acid

See Notes

0.004

Vaseline Intensive Care

Human

Dermatomed to 0.35mm

Abdomen

0.32

Flow-through

Saline

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								2.1	2.38				7

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

2.1

2.38

NOTES

Cadaver skin was used. Water permeation measurements were taken prior to the study and those with a kp greater than 2.5E-3 were discarded. 5mg/cm2 of the lotion was applied. Results were given +/- SEM. I have converted these to SD for the puropse of this database.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies V: Permeation Through Damaged Skin (1985) J. Pharm. Sci., 74 :1062-1066