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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1457

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

See Notes

0.004

Vaseline Intensive Care

Rat

Dermatomed to 0.35mm

Back

0.32

Flow-through

Saline

Skin

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								56.3	8.5				5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

56.3

8.5

NOTES

Fresh skin from 3-6 month old female osborne-mendel rats was used. 5mg/cm2 of the lotion was applied. Results were given +/- SEM. I have converted these to SD for the purpose of this database.

REFERENCE

Methods For In Vitro Percutaneous Absorption Studies V: Permeation Through Damaged Skin (1985) J. Pharm. Sci., 74 :1062-1066