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In Vitro Study No: 1464

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Dibutylphthalate10500.5291.67None (Neat)HumanIsolated EpidermisAbdomen1.8Static50% aq. Ethanol30Not Given30302Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.070.082.3E-06152.9

NOTES

The human absominal skin was obtained from cadavers. The integrity of all membranes was assessed with tritiated water on the first day of the experiment. Then 0.5ml of saline was addedto the receptor chamber and left in high humidity overnight to allow the skin to hydrate before the experiment was started. The results were given +/- SE. I have converted these to SD for the purpose of this database.

REFERENCE

In Vitro Absorption of Some o=Phthalate Diesters through Human and Rat Skin (1987) Environ. Health. Perspect., 74 :223-227