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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1469

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

DMP

1190

0.5

330.56

None (Neat)

Rat

Isolated Epidermis

Abdomen

1.8

Static

50% aq. Ethanol

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										41.6	13.22	0.000345		0.5

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

41.6

13.22

0.000345

0.5

NOTES

AL/pk Wistar-derived Rates were used. The epidermal membranes were prepared using NaBr. The integrity of all membranes was assessed with tritiated water on the first day of the experiment. Then 0.5ml of saline was addedto the receptor chamber and left in high humidity overnight to allow the skin to hydrate before the experiment was started. The results were given +/- SE. I have converted these to SD for the purpose of this database.

REFERENCE

In Vitro Absorption of Some o=Phthalate Diesters through Human and Rat Skin (1987) Environ. Health. Perspect., 74 :223-227