IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1498
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Glycerol Trioleate
0.04
0.01
0.000512
Ethanol
Mouse
Full Thickness Skin
Mid-Dorsal
0.78
Static
PBS & gentamicin
37
Receptor Fluid
24
24
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.3
0.2
6
NOTES
Hairless mouse skin was used.
REFERENCE
The In-Vitro Percutaneous Absorption of Glycerol Trioleate Through Hairless Mouse Skin (1988) J. Pharm. Pharmacol., 40 :819-821
