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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 15

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Water

0.05

0.39

0.9% Saline

Rat

Isolated Dermis

Back

0.64

flow-through

50% aq. Ethanol

31.5

Skin

Rdiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								48.1	5.7			0.0563	14	0.09

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

48.1

5.7

0.0563

0.09

NOTES

The skin was occluded with a with a glass coverslip an silicone grease to prevent evaporation. The flow rate in the cell was 1.9ml/min. The skin was taken from 4-5 wk old male Ola:Sprague Dawley rats. It was soaked in 2M Sodium Bromide for 24 hours prior to the study to separate the epidermis and dermis.

REFERENCE

Absorption Of Hydrophilic And Lipophilic Compounds Through Epidermal And Subepidermal Strata Of Rat Skin in Vitro (1998) Toxicol. Vitro, 12 :47-55