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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1501

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Glycerol Trioleate

0.04

0.01

0.000512

Ethanol

Mouse

Full Thickness Skin

Mid-Dorsal

0.78

Static

PBS, gentamicin & BSA

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								4.5	1.2				6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

4.5

1.2

NOTES

Hairless mouse skin was used. The skin was frozen fro 24h before thawing at room temperature and then mounting on diffusion cells

REFERENCE

The In-Vitro Percutaneous Absorption of Glycerol Trioleate Through Hairless Mouse Skin (1988) J. Pharm. Pharmacol., 40 :819-821