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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1509

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Coumarin

0.014

0.2

0.0044

Phosphate Buffer (pH 7.4)

Human

Full Thickness Skin

Abdomen

0.636

Static

phsphate buffer (pH 7.4)

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										0.13	0.078	0.0091	5	1.61

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.13

0.078

0.0091

1.61

NOTES

Cadaver skin was used. They did not give the concentration applied or loading. I have extrapolated it back from the flux and Kp

REFERENCE

Percutaneous Absorption of Coumarin, Griseofulvin and Propranolol Across Human Scalp and Abdominal Skin (1989) Meth. And Find Exp. Clin. Pharamcol., 11 :643-646