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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1515

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Propranolol0.0250.20.0079Phosphate Buffer (pH 7.4)HumanFull Thickness SkinScalp0.636Staticphsphate buffer (pH 7.4)37Receptor Fluid48482Radiolabelled (3H)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.04250.0110.0017511

NOTES

Cadaver skin was used. They did not give the concentration applied or loading. I have extrapolated it back from the flux and Kp.

REFERENCE

Percutaneous Absorption of Coumarin, Griseofulvin and Propranolol Across Human Scalp and Abdominal Skin (1989) Meth. And Find Exp. Clin. Pharamcol., 11 :643-646