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In Vitro Study No: 1524

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Butyl 4-hydroxybenzoate0.120.0637aq. Polysorbate 80 & polyethylene glycol 400Guinea pigFull Thickness SkinDorsal3.14Flow-throughsaline37Water Bath24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
        2.17   0.000708  

NOTES

Male Guinea Pigs were used. They also looked at how the preservative action of butylparaben is affected by different vehicles. A 1% solution of aq. Polysorbate 80 (w/v) and a 10% solution of polyethylene glycol (v/v) was used as the vehicle.

REFERENCE

Percutaneous Absorption of Butylparaben through Guinea Pig Skin In Vitro (1979) J. Pharm. Sci., 68 :596-598