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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1572

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Triclosan

Not Given

63.694

Methanol and Adhesive Durotak 387-2051

Human

Isolated Epidermis

Breast and Abdomen

0.785

Static

ethanol & PBS (25:75)

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										16.91	3.66	3.4E-05	8	1.6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

16.91

3.66

3.4E-05

1.6

NOTES

Female breast and abdominal skin was obtained from cosmetic surgery operations. The triclosan was applied from a patch so the system was occluded. 50 mg of Triclosan was used in the patch. N was not given in the paper, but as they gave both SEM and SD I was able to calculate it from those.

REFERENCE

Triclosan: Release from Transdermal Adhesive Formulations and In Vitro Permeation across Human Epidermal Membranes (2002) Int. J. Pharm., 235 :229-236