EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1583

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Thiourea

0.000198

Not Given

Ethanol

Mouse

Full Thickness Skin

Abdomen

4.9

Static

saline

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												9.6E-05	4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

9.6E-05

NOTES

Nude mice (SKH-hr-1) Concentration added was given as2.6E-6M I have converted this to mg/ml for the purpose of this database. More experimental detail is available in Ackermann C et al. Int. J. Cosmet. Sci 7 (1985) 251-264. The experiement was actually conducted for 30 hours, but this permeability coefficient was calculated from the first 6 hours of the experiment

REFERENCE

Ether-water Partitioning and Permeability Through Nude Mouse Skin in Vitro. I. Urea, Thiourea, Glycerol and Glucose (1987) Int. J. Pharm., 36 :61-66