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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1595

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Aldosterone

Not Given

Human

Isolated Epidermis

various

Static

Not Given

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												5.8E-05

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

5.8E-05

NOTES

Human cadaver skin was used. Skin was obtained form the head, chest, abdomen and back regions. The skin was stored at -80 degrees . The epidermis was obtained from the full-thickness tissue by immersion in 60 degee C watrer for 2 min.

REFERENCE

Permeation of Steriods Through Human Skin (1995) J. Pharm. Sci., 84 :1144-1146