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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1596

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Beta-Estradiol

Not Given

Human

Isolated Epidermis

various

Static

Not Given

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
												0.0041

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.0041

NOTES

Human cadaver skin was used. Skin was obtained form the head, chest, abdomen and back regions. The skin was stored at -80 degrees . The epidermis was obtained from the full-thickness tissue by immersion in 60 degee C watrer for 2 min.

REFERENCE

Permeation of Steriods Through Human Skin (1995) J. Pharm. Sci., 84 :1144-1146