IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1597
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Hydrocortisone
Not Given
Not Given
Human
Isolated Epidermis
various
Static
Not Given
37
Not Given
2
Radiolabelled (3H)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.00023
NOTES
Human cadaver skin was used. Skin was obtained form the head, chest, abdomen and back regions. The skin was stored at -80 degrees . The epidermis was obtained from the full-thickness tissue by immersion in 60 degee C watrer for 2 min.
REFERENCE
Permeation of Steriods Through Human Skin (1995) J. Pharm. Sci., 84 :1144-1146