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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1606

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Hydromorphone2.2538.599physiological phosphate bufferHumanIsolated EpidermisAbdomen0.785Staticcitrate-phosphate buffer37Water Bath56561GLC with nitrogen selective detector

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.0320.0061.5E-0549.9

NOTES

Cadaver skin was used. The skin was removed 48 hours postmortem using a dermatome set ot 0.2mm. The epidermal layers were separated by immersing each skin section in water at 60 degrees C got 30 sec. The epidermis was then tweezed with forceps. An infinte dose of hydromorphone was added therefore it was occluded in the sense that evaporation was not a factor. 4-5 studies were done. I have entered the lower number into the database.

REFERENCE

Transdermal Delivery of Narcotic Analgesics: Comparative Permeabilities of Narcotic Analgesics Through Human Cadaver Skin (1989) Pharmaceut. Res., 6 :825-832