Cadaver skin was used. The skin was removed 48 hours postmortem using a dermatome set ot 0.2mm. The epidermal layers were separated by immersing each skin section in water at 60 degrees C got 30 sec. The epidermis was then tweezed with forceps. An infinte dose of meperidine was added therefore it was occluded in the sense that evaporation was not a factor. 4-5 studies were done. I have entered the lower number into the database |