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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1617

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid 0.0050.004AcetonePigDermatomed to 1mmNot Given0.785Flow-throughTissue Culture Media Not Given24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
894  529  218   8 

NOTES

this was a low volume flow through cell and the skin was attached with a clamp but no o-ring. Flow rate was 3.5 cv/h Tissue Culture Media was RPMI-1640, Sigma st. Louis, MO

REFERENCE

A Comparison of In Vitro Skin Penetration Cells (1994) J. Pharm. Sci., 83 :1229-1233