IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1618
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Benzoic Acid
0.005
0.004
Acetone
Pig
Dermatomed to 1mm
Not Given
0.785
Flow-through
Tissue Culture Media
Not Given
24
24
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
90
5
41
5
17
9
6
NOTES
this was a high volume flow through cell and the skin was attached with a clamp but no o-ring. The flow-rate was 3.7 cv/h Tissue Culture Media was RPMI-1640, Sigma st. Louis, MO
REFERENCE
A Comparison of In Vitro Skin Penetration Cells (1994) J. Pharm. Sci., 83 :1229-1233
