EDETOX logo

IN VITRO STUDIES FURTHER DETAILS


BACK TO THE MAIN MENU

back to previous page forward to next page

 University of Newcastle homepage

In Vitro Study No: 1620

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid 0.0050.004AcetonePigDermatomed to 1mmNot Given0.785Flow-throughTissue Culture media Not Given24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
746  206  113   6 

NOTES

this was a high volume flow through cell and the skin was attached with a clamp and an o-ring. Flow rate was 1.2 cv/h. Tissue Culture Media was RPMI-1640, Sigma st. Louis, MO

REFERENCE

A Comparison of In Vitro Skin Penetration Cells (1994) J. Pharm. Sci., 83 :1229-1233