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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1622

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Beta-Estradiol

0.005

0.004

Acetone

Pig

Dermatomed to 1mm

Not Given

0.785

Flow-through

Tissue Culture Media

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
87	3			10	8			0.2					4

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

0.2

NOTES

this was ahigh volume flow through cell and the skin was attached with a clamp but no o-ring. Flow rate was 3.3 cv/h Tissue Culture Media was RPMI-1640, Sigma st. Louis, MO Results were actually given as less than 0.2% absorbed.

REFERENCE

A Comparison of In Vitro Skin Penetration Cells (1994) J. Pharm. Sci., 83 :1229-1233