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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1628

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzo[a]pyrene 0.01-0.020.0025AcetoneMouseFull Thickness Skindorsal2Flow-throughculture medium Not Given16162Radiolabelled (3H)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
94.036.61      9.172.56   10 

NOTES

Balbc Mice were used. The backs of the mice were shaved with electric clippers 24h prior to experimentation and only those mice in the resting phase of their hair cycles were used. Results were given +/- SEM. I have converted these to SD for the purpose of this database. The % absorbed included the amount recovered in the receptor fluid, filter support and the residual activity in the individual wells.

REFERENCE

In Vitro Percutaneous Absorption in Mouse Skin: Influence of Skin Appendages (1988) Toxicol. Appl. Pharmacol., 94 :93-103