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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1630

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Benzo[a]pyrene

0.01-0.02

0.0025

Acetone

Hairless Mouse

Full Thickness Skin

dorsal

Flow-through

culture medium

Not Given

Radiolabelled (3H)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
90.01	9.46							2.89	1.2				10

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

90.01

9.46

2.89

1.2

NOTES

Hairless HRS Mice were used. Results were given +/- SEM. I have converted these to SD for the purpose of this database. The % absorbed included the amount recovered in the receptor fluid, filter support and the residual activity in the individual wells.

REFERENCE

In Vitro Percutaneous Absorption in Mouse Skin: Influence of Skin Appendages (1988) Toxicol. Appl. Pharmacol., 94 :93-103