The donor compartment was covered with aluminium foil to prevent evaportation of the donor solution. The skin was preteated with Tris HCl buffer (pH 7.2) for 2 hours This was rinsed off before the benzoic acid was added. N was between 4 and 6 the skin samples were all from the same source. Methods and results were given in moles. I have converted these to grams for this database. |