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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 164

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

4-Aminobenzoic acid

0.133

0.0096

0.002

Ethanol

Guinea pig

Dermatomed to 0.2mm

Not Given

0.64

Flow-through

HHBSS

Not Given

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
				20.7	4.9	25.7	5.6	5	2.42				12

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

20.7

4.9

25.7

5.6

2.42

NOTES

Female 6 month old hairless guinea pigs were used. Skin was washed at 24h. % Absorption was given +/- SE. I have converted this to SD for this database. Potential Absorption was calculated by adding the percentage found in the receptor fluid to the percentage found in the skin after 48 hours. Some details were extracted from J.Pharm.Sci (1984) 74:64-67. Reference No:44 in this database.

REFERENCE

In Vitro Skin Absorption And Metabolism Of Benzoic Acid, p-Aminobenzoic Acid And Benzocaine In The Hairless Guinea Pig (1990) Int. J. Occup. Med. Environ. Health, 7 :1147-1151