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In Vitro Study No: 1640

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Benzoic Acid0.12210.20.02442deionised waterHumandermatomed to 0.6mmnot given1Flow-throughPBS (pH 7.4)37Water Bath48481Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          0.00740.0022 5 

NOTES

The donor compartment was covered with aluminium foil to prevent evaportation of the donor solution. The skin was preteated with Tris HCl buffer (pH 7.2) for 2 hours This was rinsed off before the benzoic acid was added. N was between 4 and 6 the skin samples were all from the same source. Methods and results were given in moles. I have converted these to grams for this database.

REFERENCE

Epidermal Enzymes as Penetration Enhancers in Transdermal Drug Delivery (1996) J. Pharm. Sci., 85 :249-252