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In Vitro Study No: 165

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
4-Aminobenzoic acid0.1330.00960.002EthanolGuinea pigDermatomed to 0.2mmNot Given0.64Flow-throughWater Not Given24482Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
   3.214.7 33.4818.716.6   12 

NOTES

Female 6 month old hairless guinea pigs were used. Skin was washed at 24h. % Absorption was given +/- SE. I have converted this to SD for this database. Potential Absorption was calculated by adding the percentage found in the receptor fluid to the percentage found in the skin after 48 hours. Some details were extracted from J.Pharm.Sci (1984) 74:64-67. Reference No:44 in this database.

REFERENCE

In Vitro Skin Absorption And Metabolism Of Benzoic Acid, p-Aminobenzoic Acid And Benzocaine In The Hairless Guinea Pig (1990) Int. J. Occup. Med. Environ. Health, 7 :1147-1151