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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1660

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Caffeine

0.016

0.1

50% aq. Ethanol

Human

Full Thickness Skin

Breast

0.64

Flow-through

saline

Receptor Fluid

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
				8.7	5.81	25.8		17.1	3.35	1.8	0.45		5	1.6

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

8.7

5.81

25.8

17.1

3.35

1.8

0.45

1.6

NOTES

Results were given +/- SEM. I have converted these to SD for the purpose of this database. After 24 hours the unabsorbed dose material was removed from the surface by swabing with 50% aq. Ethanol.

REFERENCE

Factors Affecting Percutaneous Absorption of Caffeine in Vitro (2002) Toxicology, 178 :65-66