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In Vitro Study No: 1662

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
Caffeine40.0160.150% aq. EthanolHumanDermatomed to 0.28mmBreast0.64Flow-throughsaline37Receptor Fluid24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
    3.81.7921.8 1810.733.82.68 50.9

NOTES

Results were given +/- SEM. I have converted these to SD for the purpose of this database. After 24 hours the unabsorbed dose material was removed from the surface by swabing with 50% aq. Ethanol.

REFERENCE

Factors Affecting Percutaneous Absorption of Caffeine in Vitro (2002) Toxicology, 178 :65-66