IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1665
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
Caffeine
10
0.016
0.25
50% aq. Ethanol
Human
Full Thickness Skin
Breast
0.64
Flow-through
saline
37
Receptor Fluid
24
24
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
3.8
5.81
10
6.2
8.5
18.2
1.34
5
2
NOTES
Results were given +/- SEM. I have converted these to SD for the purpose of this database. After 24 hours the unabsorbed dose material was removed from the surface by swabing with 50% aq. Ethanol.
REFERENCE
Factors Affecting Percutaneous Absorption of Caffeine in Vitro (2002) Toxicology, 178 :65-66