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IN VITRO STUDIES FURTHER DETAILS


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In Vitro Study No: 1667

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
n-Methyl-2-pyrrolidone10330.04425.825None (Neat)RatFull Thickness SkinBack1.76StaticRPMI & 1% BSA & 2% penicillin-streptomycin36Water Bath24242Radiolabelled (14C)

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
88.10.2  4.32.6978.6 74.3    6 

NOTES

Results were given +/- SEM. I have convered these to SD for this database. The maximum flux was 2100 mcrg/cm2/h. They gave the overall recovery, the unabsorbed dose and the amount recovered in the skin. I have calculated the absorbed dose from these figures.

REFERENCE

Toxicokinetics and Metabolism of N-[14C]N-Methyl-2-Pyrrolidone in Male Sprague-Dawley Rats: In Vivo and In Vitro Percutaneous Absorption (2003) Drug Met. Dispos., 31 :659-669