EDETOX logo

IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1671

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

n-Methyl-2-pyrrolidone

1033

0.704

413.2

None (Neat)

Rat

Full Thickness Skin

Back

1.76

Static

RPMI & 1% BSA & 2% penicillin-streptomycin

Water Bath

Radiolabelled (14C)

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
89.5	6.8			28.1	7.2	42.5		14.4					16

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

89.5

6.8

28.1

7.2

42.5

14.4

NOTES

Results were given +/- SEM. I have convered these to SD for this database. The maximum flux was 7500 mcrg/cm2/h. They gave the overall recovery, the unabsorbed dose and the amount recovered in the skin. I have calculated the absorbed dose from these figures.

REFERENCE

Toxicokinetics and Metabolism of N-[14C]N-Methyl-2-Pyrrolidone in Male Sprague-Dawley Rats: In Vivo and In Vitro Percutaneous Absorption (2003) Drug Met. Dispos., 31 :659-669