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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1677

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

N,N-Diethyl-m-toluamide

100

0.3

46.88

60% aq.PEG 400

Human

Full Thickness Skin

Not Given

0.64

Static

0.1M pH 7.4 PBS

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										11.95	1.42	0.00012	6	3.2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

11.95

1.42

0.00012

3.2

NOTES

Franz cells were used with a receptor volume of 4.9ml. The donor chamber was covered with a glass slip to prevent evaporation. DEET was analysed on an alltech C8 column (5 mcrl, 25cmx4.6mm) and eluted with the mobile phase at a flow-rate of 0.7 ml/min. The mobile phase was methanol/water (80:20)

REFERENCE

Reduction in Skin Permeation of N,N-diethyl-m-toluamide (DEET) by Altering the Skin/Vehicle Partition Coefficient (2000) J. Contr. Release, 67 :211-221