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In Vitro Study No: 1677

METHODS

Chemical Conc. (mg/ml)Volume (ml)Loading (mg/cm2)VehicleSpeciesMembraneSiteArea (cm2)Cell TypeReceptor FluidTemp. (oC)Temp. MeasuredExposure Time (h)Study Length (h)OccludedAnalytical Method
N,N-Diethyl-m-toluamide1000.346.8860% aq.PEG 400HumanFull Thickness SkinNot Given0.64Static0.1M pH 7.4 PBS37Water Bath36361HPLC

RESULTS

% Recovery+/-% In Stratum Corneum+/-% in Viable Skin+/-% Penetrated+/-% Absorbed+/-s-s-flux (mcrg/cm2/h)+/-kp (cm/h)nLag Time (h)
          11.951.420.0001263.2

NOTES

Franz cells were used with a receptor volume of 4.9ml. The donor chamber was covered with a glass slip to prevent evaporation. DEET was analysed on an alltech C8 column (5 mcrl, 25cmx4.6mm) and eluted with the mobile phase at a flow-rate of 0.7 ml/min. The mobile phase was methanol/water (80:20)

REFERENCE

Reduction in Skin Permeation of N,N-diethyl-m-toluamide (DEET) by Altering the Skin/Vehicle Partition Coefficient (2000) J. Contr. Release, 67 :211-221