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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1681

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

N,N-Diethyl-m-toluamide

100

0.3

46.88

30% aq. Ethanol

Human

Full Thickness Skin

Not Given

0.64

Static

0.1M ph 7.4 PBS

Water Bath

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
										124.49	47.15	0.00121	6	13.2

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

Lag Time (h)

124.49

47.15

0.00121

13.2

NOTES

Franz diffusion cells were used. The donor compartment was covered with a glass slip to prevent evaporation. DEET was analysed on an alltech C8 column (5 mcrl, 25cmx4.6mm) and eluted with the mobile phase at a flow-rate of 0.7 ml/min. The mobile phase was methanol/water (80:20)

REFERENCE

Percutaneous Permeation of N,N-Diethyl-m-Toluamide (DEET) from Commercial Mosquito Repellents and the Effect of Solvent (1997) J. Toxicol. Environ. Health, 52 :119-135