IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 169
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
2-Phenoxyethanol
21.6
0.01
0.272
Methanol
Rat
Dermatomed to 0.244mm
Dorsal
0.79
Static
50% aq. Ethanol
37
Receptor Fluid
24
24
2
Radiolabelled (14C)
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
67.6
0.9
0.2
1
0.2
65.9
64
9.8
53.47
16.17
0.00272
6
0
NOTES
The rats were Male Wistar Rats and hair was removed from the dorsal skin with clippers. Volume added was given as 1556 nmol or 0.81mCi. Sp Activity was 3.74mCi/mg. The results were given as +/- SEM. I have converted this to SD for this database.
REFERENCE
Percutaneous Penetration Of 2-Phenoxyethanol Through Rat And Human Skin (1997) Food Chem.Toxicol., 35 :1009-1016
