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IN VITRO STUDIES FURTHER DETAILS

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In Vitro Study No: 1695

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

0.03

0.32

0.015

PBS/EtOH 95:5

Human

Full Thickness Skin

abdomen, thigh, upper arm

0.64

Static

0.9%NaCl

34

Skin

24

24

2

GCMS

RESULTS

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

n

Lag Time (h)

37.6

1.6

0.3

0.02

0.8

NOTES

REFERENCE

Percutaneous absorption of aromatic amines – A contribution for human health risk assessment (2008) Food Chem.Toxicol., 46 :1960-1968