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IN VITRO STUDIES FURTHER DETAILS

In Vitro Study No: 1706

METHODS

Chemical

Conc. (mg/ml)

Volume (ml)

Loading (mg/cm2)

Vehicle

Species

Membrane

Site

Area (cm2)

Cell Type

Receptor Fluid

Temp. (oC)

Temp. Measured

Exposure Time (h)

Study Length (h)

Occluded

Analytical Method

Methiocarb

0.045

0.6

0.013

Mesurol in 0.9% NaCl in water plus 2% ethanol

Human

Full Thickness Skin

Breast and abdomen

2.12

Static

0.9% NaCl

32

Skin

24

1

HPLC

RESULTS

% Recovery	+/-	% In Stratum Corneum	+/-	% in Viable Skin	+/-	% Penetrated	+/-	% Absorbed	+/-	s-s-flux (mcrg/cm2/h)	+/-	kp (cm/h)	n	Lag Time (h)
								15.2	1.6	0.1	0.01			9.8

% Recovery

+/-

% In Stratum Corneum

+/-

% in Viable Skin

+/-

% Penetrated

+/-

% Absorbed

+/-

s-s-flux (mcrg/cm2/h)

+/-

kp (cm/h)

n

Lag Time (h)

15.2

1.6

0.1

0.01

9.8

NOTES

Exposure in methods is 30 h. Mesurol contains 1% w/w methiocarb.

REFERENCE

Percutaneous penetration through slightly damaged skin (2005) Arch. Dermatol. Res., 296 :560-567