IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1708
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
o-toluidine
1008
0.32
504
undiluted
Human
Full Thickness Skin
Abdomen and thigh
0.64
Static
0.9% NaCl
34
Skin
4
4
1
GCMS
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.04
0.01
66.6
18.9
1.4
NOTES
REFERENCE
Enhancement of percutaneous penetration of aniline and o-toluidine in vitro using skin barrier creams (2008) Toxicol. Vitro, 22 :812-818