IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1710
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
o-toluidine
1000
0.32
500
undiluted
Human
Full Thickness Skin
Abdomen, thigh, upper arm
0.64
Static
0.9% NaCl
34
Skin
8
8
1
GCMS
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
0.08
0.02
117
28.3
2.5
NOTES
REFERENCE
Percutaneous absorption of aromatic amines – A contribution for human health risk assessment (2008) Food Chem.Toxicol., 46 :1960-1968
