IN VITRO STUDIES FURTHER DETAILS
In Vitro Study No: 1711
METHODS
Chemical
Conc. (mg/ml)
Volume (ml)
Loading (mg/cm2)
Vehicle
Species
Membrane
Site
Area (cm2)
Cell Type
Receptor Fluid
Temp. (oC)
Temp. Measured
Exposure Time (h)
Study Length (h)
Occluded
Analytical Method
o-toluidine
0.03
0.32
0.015
PBS/EtOH 95:5
Human
Full Thickness Skin
Abdomen, thigh, upper arm
0.64
Static
0.9 NaCl
34
Skin
24
24
1
GCMS
RESULTS
% Recovery
+/-
% In Stratum Corneum
+/-
% in Viable Skin
+/-
% Penetrated
+/-
% Absorbed
+/-
s-s-flux (mcrg/cm2/h)
+/-
kp (cm/h)
n
Lag Time (h)
49.8
3.2
0.37
0.04
0.8
NOTES
REFERENCE
Penetration of β-naphthylamine and o-toluidine through human skin in vitro (2006) Arch. Toxicol., 80 :644-646
